4/29/2023 0 Comments Find stop codons snapgenecoli, deleting the floxed portion of a construct during the cloning process). This alteration enables the generation of a single vector containing Cre and a floxed construct (unmodified Cre will cause recombination in E. CREM (or Cre-M) has been engineered to contain an intron, preventing Cre expression when cloning in E. ![]() Optimized Cre: Codon-optimized Cre ( iCre) is expressed at higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven recombination.Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression.Cre may be expressed globally under a broadly active promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Promoter-regulated Cre: The promoter region defines the areas in which Cre will be expressed.One advantage of this system is tight temporal regulation. Inducible Cre: These constructs require the addition of an exogenous ligand (e.g.Addgene has a wide variety of Cre recombinase plasmids. ![]() Scientists have developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Read Addgene's Blog on Cre alternatives from Gerald Rubin's lab. While Cre-lox recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. ![]() Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ and Cre- cells. By expressing Cre at specific times or locations, you can precisely control expression of your gene of interest. For a more thorough introduction, check out Addgene’s Cre-lox blog post.īased on these Cre-lox recombination principles, scientists have developed constructs to activate/inactivate genes when Cre is present.
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